CryptococcusneoformansIndiainkstaining.jpg (800 × 536 pixels, file. Often occurs in immunocompromised individuals: Infection with this pathogen is heavily associated with AIDS infection (CD4 count < 100 cells/mm3), those with hematological malignancy, and those on high dose steroid therapy. India ink staining of the CSF was performed by the referring laboratories and repeated by the reference laboratory on available specimen (cultured isolate. File:Cryptococcus neoformans India ink staining.jpg. It belongs to the class of drugs of azoles. It is used with amphotericin B to treat infection with Cryptococcus neoformans.įluconazoleis often used in AIDS patients with more mild Cryptococcus neoformans infections. neoformans and only useful for static observations of the capsule. with CSF examination aand cryptococcal antigen assay (or India ink if. This makes India ink formulations toxic for C. Cryptococcus neoformans infections from Cryptococcus gattii have been reported. This agent is used to treat infection with Cryptococcus neoformans (especially in the case of cryptococcal meningitis).įlucytosineis an agent that converts inhibits DNA and RNA biosynthesis. Despite its usefulness as a diagnostic technique, the India ink procedure has a major disadvantage for capsule research: commercial vendors add preservatives, such as thimerosal, to the ink preparation. Hematogenous spread to meninges CONDITIONS CAUSEDĭermatological: Wound/cutaneous cryptococcosis TREATMENTĪmphotericin Bis an antifungal agent that binds ergosterol (which is unique to fungus) and forms membrane pores that allow leakage of electrolytes. Inhalation of spores from the environment (distribution is worldwide in the soil, also found in pigeon droppings).
Urease positive: this pathogen produces urease. Polysaccharide capsular antigen can be detected in the lab using a latex agglutination test.MOLECULAR CHARACTERISTICSĬapsule:this fungi has a thick outer capsule composed of polysaccharides. It employs 2 chromium mercury and India ink. India ink stain of Cryptococcus neoformans ( source) CULTURE CHARACTERISTICSĬultured on Sabouraud agar. A novel modified India ink technique for the diagnosis of Cryptococcus neoformans in cerebrospinal fluid specimens is described. Mucicarmine can also be used to stain the capsule of the fungi red. Cryptococcus neoformansis a yeast fungus that can be visualized with an India ink stain due to its thick capsule (which prevents the fungi itself from being stained).
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